Effectiveness of stevia rebaudiana whole leaf extract against. In this report, stevioside and steviol were tested for mutagenicity in salmonella typhimurium strains ta98 and ta100 and for chromosomal effects on cultured human lymphocytes. Characterization and quantification of hydroxycinnamate. The leaves of this plant has a pleasantly sweet and refreshing taste which is educe by diterpene glycosides stevioside and rebaudiosides, a highpotency sweeteners and. Influence of nacl on biochemical parameters of two. Pdf on jul 20, 2017, manvender singh and others published in vitro propagation of stevia rebaudiana bertoni. It is now grown in other parts of the world, including canada and part of asia and europe. However, it is popular as a sugar substitute, and the glycosidal diterpens in the leaves are 30 times sweeter than sugar. The present study aims at developing a reliable protocol for in vitro propagation and cryostorage shoot tips of stevia rebaudiana using a vitrification method. Lingstrom b, d a department of health sciences, and b who collaborating centre for epidemiology and community dentistry.
In vitro antioxidant activities of stevia rebaudiana. Two node microcuttings were subjected to ms media supplemented with different nacl concentrations 0, 25, 50, 75, 100, 125 mm. Comparative studies of in vitro and in vivo grown plants and. The extract is about times sweeter than sugar so it can be used at a very low concentration. Pdf in vitro propagation of stevia rebaudiana bertoni. Stevia is native to paraguay south america and also called sweet herb of paraguay.
Leaf extract of stevia rebaudiana promotes effects on certain physiological systems such as the cardiovascular and renal and influences hypertension and hyperglycemia. Studies on rapid micropropagation of stevia rebaudiana. Since these activities may be correlated with the presence of antioxidant compounds, leaf and callus extracts of stevia rebaudiana were evaluated for their total phenols, flavonoids content and total antioxidant cap. Considering the effectiveness of stevia leaf extract in laboratory and clinical studies, we evaluated the antimicrobial potential of stevia whole leaf extracts against the lyme disease causing pathogen, b. Effectiveness of stevia rebaudiana whole leaf extract. Production of steviosides in ex vitro and in vitro grown. For invitro regeneration of stevia rebaudiana bertoni murashige and skoogs medium was used with various concentrations of plant growth regulators 7. Family asteraceae is a natural sweetener plant that is grown commercially in many parts of brazil, paraguay, central america, thailand, korea, china and india. Twentyfour hydroxycinnamic acid derivatives of quinic and shikimic acid were detected, and 19 of them were successfully. Nov 12, 2015 considering the effectiveness of stevia leaf extract in laboratory and clinical studies, we evaluated the antimicrobial potential of stevia whole leaf extracts against the lyme disease causing pathogen, b. Stevia stevia rebaudiana is a bushy shrub that is native to northeast paraguay, brazil and argentina. The protocol for in vitro propagation of stevia rebaudiana is scanty sivaram and mukundan, 2003. Stevia rebaudiana leaves were obtained from anna medicinal farm, chennai. In vitro callusing in stevia rebaudiana bertoni using cyanobacterial mediaa novel approach to tissue culture.
An efficient high frequency plant regeneration protocol through direct organogenesis was developed for sevia rebaudiana. An in vitro and in vivo comparison of the effect of stevia. Stevia rebaudiana has been used in numerous countries for thousands of years as a source of sweetener that is all natural and will not raise a persons glycemic index, which is. Qualitative composition of the sgs in in vitro plants was found to be identical to that of intact plants, but their content in the former plants appeared to be about five or six times lower. For invitro regeneration of stevia rebaudiana bertoni murashige and skoogs medium was used with various concentrations of plant growth regulators7. Effect of different levels of farmyard manure and nitrogen on the yield and nitrogen uptake by stevia stevia rebaudiana bertoni. Nutrient factors affecting in vitro cultivation of stevia rebaudiana. It has elongated leaves that grow along the stems and are lined up against each other. The study included the effect of ms salt strength and major elements containing medium at different levels on the in vitro growth and development of stevia rebaudiana the best nutrient medium was ms at full salt strength or modified ms major elements at these levels nh4no3 at 1237. Stevia rebaudiana sr is often used by the food industry due to its steviol glycoside content, which is a suitable caloriefree sweetener.
Stevia rebaudiana, bertoni is a small shrub belong to family. Pdf in vitro propagation of stevia rebaudiana bertoni in. Shoot apex, nodal, and leaf explants of stevia rebaudiana bertoni can regenerate shoots when cultured on murashige and skoog ms medium supplemented with 6benzyladenine ba. Stevia rebaudiana leaves are used as a zerocalorie natural sweetener in a variety of food products in asian countries, especially in japan. An efficient stevia rebaudiana transformation system and in.
Stevia rebaudiana bertoni plants grown in vitro and ex vitro were investigated for variations in the profile of steviosides in their leaves, shoots, roots and flowers. In vitro culture studies on stevia rebaudiana request pdf. Qualitative composition of the sgs in in vitro plants was found to be identical to that of intact plants, but their content in the former plants. In vitro propagation of stevia rebaudiana bert in bangladesh. Pdf in vitro micropropagation of stevia rebaudiana bert. In vitro propagation of stevia rebaudiana semantic scholar. Since these activities may be correlated with the presence of antioxidant compounds, leaf and callus extracts of stevia rebaudiana were evaluated for their total phenols, flavonoids content and total antioxidant capacity. Pdf an efficient protocol for in vitro regeneration of.
In vitro shoot multiplication in stevia rebaudiana bert. To effectively evaluate the whole stevia leaf extract, we. Stevia is sold as a concentrated white stevia stevia rebaudiana powder to sprinkle on food. In vitro antioxidant activities of stevia rebaudiana leaves. Around 82% of plants were successfully established in natural field condition. In vitro micropropagation of stevia rebaudiana bertoni in.
The present study was aimed at the developing an efficient protocol for successful in vitro rooting of stevia shoot fragments and subsequent acclimatization of. Comparative studies of in vitro and in vivo grown plants. It is a small seasonal plant which grows to a height of 12 feet 3061 cm. Materials and methods plant material stevia rebaudiana plants were collected from horticultural institute, yercaud, tamilnadu, india and maintained in green house. Uses, side effects, interactions, dosage, and warning. Stevia rebaudiana bertoni, known to be an antidiabetic medicinal herb, belongs to asteraceae family which has bioactive compounds stevioside and rebaudioside in its leaves that taste about 300350 times sweeter than sucrose.
Antioxidants free fulltext antioxidant metabolism and. Comparative studies of in vitro and in vivo grown plants and callus of stevia rebaudiana bertoni. This plant is recorded as having a noncaloric natural sugar, alternative to artificially. An efficient stevia rebaudiana transformation system and. Stevia rebaudiana bertoni is one of a very few plant species that produce zero calorie, sweet compounds known as steviol glycosides sg. Morphoanatomical study of stevia rebaudiana roots grown in. Rooting of the in vitro derived shoots could be achieved following subculture onto auxincontaining medium. It is mainly used as a sweetener and flavor enhancer in the food and beverage industry. Shoot tips containing axillary buds were used as an explant and inoculated on murashige and skoogs ms medium containing 3%. Optimization of micronutrients for the improvement of in vitro plant regeneration of stevia rebaudiana bert. Stevia stevia rebaudiana bertoni is a perennial shrub belonging to the asteraceae family. In the present study, a protocol was developed for in vitro micropropagation using 6benzylamino purine bap and kinetin kn for the formation of multiple shoot proliferation and indole3acetic acid iaa, indole3butyric acid iba and 1naphthaleneacetic acid naa.
A survival rate of 70% was recorded at the hardening phase on the. Adaptation to ex vitro conditions of stevia rebaudiana. Stevia rebaudiana bertoni, belonging to family asteraceae and natural sweet plant, but due to poor seed viability, fertility and vigor, stevia cultivation is a challenging task. Stevia rebaudiana bertoni is a medicinal plants and commercially use as noncaloric sweetener for diabetic patient. Stevia rebaudiana extract is often referred to as stevia. It is commonly known as candyleaf, sweetleaf or sugarleaf it is a small seasonal plant which grows to a height of 12 feet 3061 cm.
Stevia rebaudiana bertoni is a medicinal plants and commercially use as non caloric sweetener for diabetic patient. In vitro multiplication of stevia rebaudiana article pdf available in current science 7011. In vitro propagation of stevia rebaudiana bertoni in egypt. Preparation of plant extract 25 g of airdried powder of stevia rebaudiana leaves was immersed in 100 ml of organic. The leaves of stevia are the source of sweet glycosides.
Keeping in view the importance of stevia and salinity, present study had been designed to investigate the effect of salinity on biochemical parameters in two stevia genotypes. It is commonly known as candyleaf, sweetleaf or sugarleaf. Stevia is an erect, taproot and perennial herbaceous species that reaches up to 90 cm in height, with small leaves that measure from 3 to 5 cm in length and 1 to 1. Leaves of stevia rebaudiana bertoni have been popularly used as a sweetener in foods and beverages for diabetics and obese people due to their potent sweetener stevioside. According to the extent of lipid peroxidation, an oxidative stress occurred during the first hours of acclimatisation. Invitro antimicrobial and antitumor activities of stevia. An overview find, read and cite all the research you need on researchgate. Stevia has garnered attention with the rise in demand for lowcarbohydrate, lowsugar food alternatives. Stevia stevia rebaudiana bertoni, with great potential as a natural sweeteners source, has a high content of sweeteners, which are up to 150 times sweeter than sugar, but virtually with no calories. The purpose of the study is to evaluate the antimicrobial and antitumor activities of stevia rebaudiana asteraceae leaf extracts. In vitro shoot multiplication of stevia rebaudiana, an. Studies on rapid micropropagation of stevia rebaudiana bertoni.
An overview manvender singh, vinod saharan, jyotsna dayma, deepak rajpurohit, yadunandan sen and ajay sharma department of molecular biology and biotechnology, rajasthan collage of agriculture, maharana pratap university of agriculture and technology, udaipur, rajasthan, india. Stem cutting propagation for exsitu conservation of. Further, both in vitro and in vivo studies indicate that these glycosides and the extracts from sr have pharmacological and therapeutic properties, including antioxidant, antimicrobial, antihypertensive, antidiabetic, and anticancer. An efficient plant regeneration protocol has been developed from leaf explants of stevia rebaudiana, an economically and medicinally important. A survival rate of 70% was recorded at the hardening phase. Pdf in vitro culture studies on stevia rebaudiana re. In this study, the functioning of antioxidant metabolism and photosynthesis efficiency during the acclimatisation of stevia rebaudiana plants to ex vitro conditions was determined. Indian journal of biotechnology vol 11, october 2012, pp 486490 optimization of micronutrients for the improvement of in vitro plant regeneration of stevia rebaudiana bert. In vitro rapid multiplication of stevia rebaudiana. Stevia rebaudiana bertoni is a medicinal plant belongs to asteraceae family and known as stevia, sweet leaf, honey leaf, and. This plant has been used in folk medicine to treat hypertension, diabetes and as a contraceptive.
Nutrient factors affecting in vitro cultivation of stevia. Stevia rebaudiana bertoni bertoni, asteraceae asterales, has long been known to the indians, who call it kaa hee sweet herb. In this study, the hydroxycinnamate derivatives of s. Steviosides were extracted by hydrolysis and esterification, evaporated to dryness and dissolved in methanol for quantitative analysis by high. In vitro mineral nutrition for improving growth and. Optimization of micronutrients for the improvement of in. Impact of glycine betaine on salinity tolerance of stevia. Pdf feas elarish, north sinai, suez canal universityscu and biotechnology laboratory in north sinai station, desert research centerdrc cairo. S rebaudiana is originally a south american wild plant katayma et al. Influence of nacl on biochemical parameters of two cultivars of stevia rebaudiana regenerated in vitro. Stevia rebaudiana is a shrublike plant in the sunflower family that is native to north and south america and is known for its sweet leaves. Mutagenicity and human chromosomal effect of stevioside, a. In vitro culture studies on stevia rebaudiana springerlink. In vitro propagation is important for rapid multiplication of a wide range of nursery crops or medicinal plants, including stevia stevia rebaudiana bertoni.
Response surface methodology was used to design experiments by varying three factors. In the present study, a protocol was developed for in vitro micropropagation using 6benzylamino purine bap and kinetin kn for the formation of multiple shoot proliferation and indole3acetic acid iaa, indole3butyric acid iba and 1naphthaleneacetic acid. The present study was aimed at the developing an efficient protocol for successful in vitro rooting of stevia shoot fragments and subsequent acclimatization of regenerants to ex vitro conditions. Stevia rebaudiana bertoni is a small perennial shrub of the asteraceae compositae family that is native to paraguay, brazil, and argentina. Pdf micropropagation of stevia rebaudiana in vitro researchgate. The active compounds are steviol glycosides mainly stevioside and rebaudioside, which have 30 to 150 times the sweetness of sugar, are heatstable, phstable, and not fermentable.
Stevia rebaudiana is a plant species in the genus stevia of the sunflower family. Singlenode shoot fragments obtained from plants propagated on ms medium were placed onto media supplemented with. Rooting of the in vitroderived shoots could be achieved following subculture onto auxincontaining medium. In vitro culture is a technique that allows studying the behavior of agnps in plants of agricultural interest such as stevia stevia rebaudiana b. Cultivation and uses of stevia stevia rebaudiana bertoni. Stevia is an extract from stevia rebaudiana, having up to 300 times the sweetness of sugar.
Bertoni pourvi jain, sumita kachhwaha and s l kothari department of botany, university of rajasthan, jaipur 302 004, india received 29 october 2011. Bertoni pourvi jain, sumita kachhwaha and s l kothari. Stevia rebaudiana bertoni is a medicinal plant belongs to asteraceae family and known as stevia, sweet leaf, honey leaf, and candy leaf. Antioxidant metabolism and chlorophyll fluorescence during.